Crystallization of Membrane-bound Alcohol Dehydrogenase of Acetic Acid Bacteria

Pentose oxidation by acetic acid bacteria led to a finding of membrane-bound purine nucleosidase.

D-Ribose and 2-deoxy-D-ribose were oxidized to 4-keto-D-ribonate and 2-deoxy-4-keto-D-ribonate respectively by oxidative fermentation, and the chemical structures of the oxidation products were confirmed to be as expected. Both pentoses are important sugar components of nucleic acids. When examined, purine nucleosidase activity predominated in the membrane fraction of acetic acid bacteria. This...

Deoxyribonucleic Acid Hybrids of Acetic Acid Bacteria.

De Ley, J. (State University, Ghent, Belgium), and S. Friedman. Deoxyribonucleic acid hybrids of acetic acid bacteria. J. Bacteriol. 88:937-945. 1964.-Deuterated N(15)-labeled deoxyribonucleic acid (DNA) from Acetobacter aceti (mesoxydans 4) forms hybrids with ordinary DNA from other species of this genus (A. xylinum, A. pasteurianus, A. estunensis, and possibly A. xylinoides) when the guanine ...

Membrane-bound alcohol dehydrogenase is essential for glyceric acid production in Acetobacter tropicalis.

Acetobacter tropicalis NBRC16470 can produce highly enantiomerically pure D-glyceric acid (D-GA; >99 % enantiomeric excess) from glycerol. To investigate whether membrane-bound alcohol dehydrogenase (mADH) is involved in GA production in A. tropicalis, we amplified part of the gene encoding mADH subunit I (adhA) using polymerase chain reaction and constructed an adhA-disrupted mutant of A. trop...

Coenzyme linked dehydrogenases of acetic acid bacteria.

Utilization of ethanol by acetic acid bacteria.

It is well known that the acetic acid bacteria can rapidly oxidize ethanol to acetic acid. But whether ethanol can serve as a source of carbon and energy for these bacteria has not been clearly established. Pasteur (1868) noted that Mycoderma aceti which is undoubtedly Acetobacter aceti (Frateur, 1950) can grow well in ethanol media only if acetic acid is also present. Addition of one or two pe...